GI muscle contractility

GI muscle contractility

GI contractility studies are useful in determining how compounds affect GI motility in either a therapeutic capacity (evaluation of drugs for motility disorders) or from a safety perspective. Although there has been much progress in the diagnosis and therapy of these diseases, curative treatment options are still limited and long-term outcome often remains unsatisfactory.

The contractile properties of GI smooth muscle can be assessed using in vitro preparations of nearly all the GI tract regions including esophagus, lower esophageal sphincter, stomach, small intestine or colon. Full thickness GI muscle strips can be dissected to obtain either circular or longitudinal muscle strips and both direct responses (agonist and antagonist effects on contraction and relaxation) and modification of nerve-evoked responses using electrical field stimulation, can be evaluated.

Figure 1. Carbachol causes a concentration-dependent constriction of human colonic muscle strips.

 

Figure 2. Carbachol enhances the magnitude of the EFS induced contractions of human colonic muscle strips orientated in both the circular and longitudinal plains.

 

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