Efficacy - Skin

Efficacy - Skin

Biopta is able to research many functions in human skin and subcutaneous tissues.  One of these key areas is the assessment of anti-inflammatory activity in fresh full thickness ex vivo human skin.  We are able to source healthy fresh human skin several times a week, enabling us to rapidly assess your novel compounds and provide invaluable human efficacy data.

 

Inflammation Model

Our inflammation model is an ideal method of assessing the anti-inflammatory properties of your test compound in vitro, up to 20 conditions can be assessed using skin from a single donor allowing for a comparatively high throughput and quick turnaround.  Test article can be added to the culture media or applied topically and the resultant changes in cytokines and other inflammatory mediators can be assessed by multiplex ELISA.

An acute inflammatory response is simulated in healthy, full thickmess, human skin biopsies, sourced from cosmetic surgery procedures, using LPL, PHA or UV light. Inflammatory mediators and cytokines such as IL-6, IL-8 and IL-10 are increased and the anti-infammatory effects of your test compound can be assessed by measuring the resultant effects on these inflammatory mediators.

Above is some sample data from our validation with dexamethasone and triamcinolone, where the creams were applied topically to skin samples which had been stimulated with LPL. As you can see, each of the anti-inflammatory creams had the desired response.

It is possible to obtain bewteen 10 and 20 separate biopsies from each individual donor, measning several compounds can be screened simultaneously for anti-inflammatory effects making the assay comparatively high throughput.

This same model can also be used to assess the inflammatory effect of test articles.  As the assay is viable for up to 7 days, repeated application of test article can be made and resultant cytokine changes can be assessed by multiplex ELISA.
 

Contact Biopta at info@biopta.com to discuss a study outline with one of our expert scientists.

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